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EBO2-Yu Ozone Method

Extracorporeal Blood Oxygenation and Ozonation developed by Dr. Yu

Also known as ozone dialysis and developed by Louie Yu, PhD, the EBO2-Yu method is the newest and most comprehensive protocol for administering ozone and purifying a patient’s blood that is available in a clinical setting today. It is the closest thing to an oil change for your body! EBO2 is an absolute breakthrough in the field of biotechnologies that is exploding in popularity all over the world within the functional and regenerative medicine community.

Similar to other ozone delivery methods, (such as “Ten Pass Ozone”), the EBO2-Yu method is a protocol of infusing ozone into your blood outside the body and then infusing it back into the patient intravenously. However, unlike the Ten Pass Ozone method of taking out a small amount of blood a handful of times and exposing it to ozone alone, the EBO2-Yu method pulls out and purifies the entire blood supply of the patient’s body by running it through a dialysis filter (ergo, “ozone dialysis”, please note: because we only add ozone gas to the blood and not large volumes of water as is done in traditional dialysis, ozone dialysis does not carry the risks of traditional dialysis)

The EBO2-Yu method combines ozonation with two other components that take the effectiveness to the next level.

The entire process is comprised of 4 main steps:

Ultraviolet Blood Irradiation

Comprehensive Filtration


Red and Far Infrared Light Therapy

1. Ultraviolet Blood Irradiation(UBI):

Following ozonation, the blood is exposed to a high powered ultraviolet light, a process known as Ultraviolet Blood Irradiation (UBI). Similar to ozone, ultraviolet light also acts as a powerful oxidant in the blood. The specific oxidative characteristic of Ultraviolet light has been shown to stimulate various types of immune cells and enhance their capacity to neutralize excess free-roaming pathogens, toxins, excess blood lipids, and metabolic waste products.

Also similar to ozone, UBI provides its own specific brief burst of oxidative stress that also stimulates a powerful adaptive response in the blood. This response triggers the up-regulation of many antioxidant defenses that lead to decreased systemic inflammation and increased tissue oxygenation. Historically UBI was actually a highly accepted, virtually side-effect-free treatment for infections until the 1940s and 1950s when its popularity was completely eclipsed by the debut of penicillin antibiotics and the Salk polio vaccine. In fact, within certain scientific circles, UBI has often been referred to as “the cure that time forgot” because of its profound ability to successfully treat a wide variety of infections without any reports of the pathogens developing a resistance to the therapy over time, especially appealing in this day and age.

After the blood has been through the three steps of filtration, ozonation, and irritation with ultraviolet light, the newly purified blood runs into the tube connected to the other arm and is immediately infused back into the patient’s body. The difference in color is striking and satisfying to observe. The “old” oxygen-poor blood is very dark red, while the “new” oxygen and ozone-rich blood is very light red.

2. Comprehensive Filtration:

During the EBO2-Yu protocol, the patient has tubes hooked up to each arm intravenously. Blood is pulled out of one arm with a very specific peristaltic pump and is first run through a special patented filtration system (called the Elisio 9H hemo dialyzer). This dialyzer filter has hollow fibers, which means that the filters themselves are made up of thousands of tiny straws which act as filtration membranes and the distinct material is polyethersulphone (PES) which is a class A resistant ozone material. Additionally, the Elisio Nepro 9H dialyzer that is used is BPA and DEHP free which differentiates it from other available dialysis filters on the market.

As the blood passes through the filter it removes:

Osteoarthritis of any joint

fats, cholesterol

heavy metal toxins

cellular waste senescent cells (old and malfunctioning cells)

Removing these particles in and of itself may help to reduce systemic inflammation in the patient that contributes to chronic disease and accelerated aging.

The filtered waste is collected in a receptacle and appears as a yellowish fluid at the bottom covered with a white foam on top. Seeing the waste collect is striking and satisfying to observe. ​

We believe this is an excellent step to “prepare the soil” immediately before a stem cell treatment to avoid inflammatory flare-ups and optimize outcomes.

3. Ozonation:

The blood then passes through another chamber where it is saturated with ozone, which is part of the device’s gas exchange filter. There are volumes of scientific literature spanning over decades which demonstrate that ozone is a very potent oxidant, which has the ability to neutralize virtually any pathogen (virus, bacteria, fungi) that it comes in contact with.

After the ozone is diffused into the blood, it elicits its pathogen killing antiseptic effect almost immediately as it begins to dissipate within seconds. It then immediately forms secondary metabolites as byproducts called Ozonides. Ozonides consist of special types of reactive oxygen species (such as peroxides and aldehydes) that travel through the blood and act as signaling molecules for the immune system and various other adaptive processes in the body when the blood is eventually reintroduced into the patient. These signaling molecules induce a mild amount of “hormetic” stress, which activates the body’s core adaptive response and fuels the communication network that regulates the balance between its immune and antioxidant systems.

This amplifies the body’s ability to produce many compounds it needs to fight off infections, neutralize remaining toxins and reduce excess inflammation. Ozonides also allow the body to produce a compound called 2,3-diphosphoglycerate (2,3-DPG) in red blood cells, which causes more oxygen to be released by hemoglobin. This super oxygenates the blood, which further enhances the cleansing process of the blood and allows all of its cells to function more efficiently.

4. Red/Far Infrared Light Therapy

In the final step, the blood is bathed in red and far infrared light. Light therapy is a well-researched and widely used therapy treatment. Hospitals have long used blue light to treat jaundice in premature infants in order to protect them from brain damage, while physical therapists often use infrared light on an injured knee or other body part to increase blood flow and nourish tissues with oxygen.

Research from Russian biophysicist Tiina Karu, Ph.D., of the Laser Technology Center in Moscow, shows that red light affects our bodies at the cellular level. Various wavelengths of red light easily penetrate the skin and stimulate energy production within the mitochondria, the energy-producing powerhouses of the cells. This helps your body create energy, vitality, and stamina, so every tissue and organ system can run more efficiently.

Red light is profoundly anti-aging and promotes health, strength, and vitality for virtually every organ in the body. More specifically, red light has been used to heal bone conditions such as osteoarthritis and fractures. It also helps to relieve pain and inflammation in joints, muscles, and tendons, and stimulates the healthy circulation of blood to tissues. Red light has been shown to heal repetitive stress syndrome such as carpal tunnel syndrome, and skin conditions such as varicose vein ulcers. Plus, it improves blood flow to the heart, eases migraine headaches, improves energy levels in people with chronic fatigue.